More Essay Examples on Enzyme Rubric In agarose gel electrophoresis, a molecular sieve is created such that the distance traveled in the gel toward the anode by any DNA fragment all of which carry negatively charged phosphate groups in the presence of a basic buffer is inversely proportional to its molecular weight. Since DNA consists solely of deoxyribonucleotides that differ only by their bases and each base pair has approximately the same molecular weight, the distance traveled in the gel toward the anode by any DNA fragment also has a linear relationship with the log of its length in base pairs.
Metabolism The major objective of this experiment was to observe the effects of catalase under varying controlled conditions.
The scope of this experiment includes Metabolic processes, such as cellular respiration, and it poisonous byproduct hydrogen peroxide. The methodology includes procedures; multiple variables were tested in specific concentrations; that test the reaction rates of the enzyme catalase over a fixed period of time.
We will write a custom essay sample on Enzyme Lab Report or any similar topic specifically for Enzyme lab report essay example Do Not Waste HIRE WRITER The major conclusion was that catalase reacts faster in warm temperatures that are neither freezing nor boiling, catalase performs well in lower concentrations than the substrate, and catalase prefers neutral pH levels around 7.
Introduction Enzymes are proteins that catalyze metabolic reactions vital for the survival and functioning of cells .
Without enzymes, metabolic processes would occur at unfeasible rates. Catalase is a naturally occurring enzyme that breaks down hydrogen peroxide into water and oxygen; it is essential to cellular respiration.
I asked if enzyme activity was affected when exposed to different conditions, such as temperature, substrate concentration, and pH levels. My first hypothesis is that higher temperatures amplify catalyzation, although too high of a temperature denatures enzymes.
My second hypothesis is that smaller concentrations of enzyme in the enzyme to substrate concentration ratios produce higher reactions. The last hypothesis is that enzyme reactions work best at a neutral pH. I observed and recorded the effect of these conditions on catalase.
The enzyme catalase is important because it handles the decomposition of approximately half of generated H2O2 in living organisms . It is important for biologists to understand catalase because all cells produce hydrogen peroxide, and its the job of catalase to break it down. This research will help increase the knowledge of enzyme activity in complex environments, and under what conditions the enzyme catalase will perform best.
The materials used in this experiment included 4 test tubes 5 for pHatalase, hydrogen peroxide, an ice water bath, a warm water bath, a boiling water bath, thermometer, test tube rack, a wax pencil, a metric ruler, a timing device, and pH solutions at 1, 4, 7, 10 and Three different experiments were tested in this lab; temperature, substrate concentration and pH.
The positive control of this experiment was the test tube kept in room temperature, and the negative control was the test tube placed in the ice. The independent variables of this experiment were time and temperature, and the dependent variable was the amount of reaction that occurred.
Each test tube was left at the designated temperature for two minutes, and then afterwards were tested every ten seconds for a minute. My technique included swirling the test tube 3 times each after every ten seconds, and then I would record the bubble height in centimeters.
In the Substrate concentration experiment, I filled the test tubes with different enzyme to hydrogen peroxide concentrations, which were measured in centimeters.
Next, I labeled the test tubes accordingly; 1 1 cm: The positive control of the experiment was the test tube with the 1: The independent variables were time and the ratio of enzyme to substrate concentration and the dependent variable was the amount of reaction that occurred.
Each test tube was left at room temperature and then tested every ten seconds for a minute. The technique used was the same as experiment one. In the pH experiment, I filled all 5 of the beakers with 2 centimeters of Catalase and 3 centimeters of hydrogen peroxide.
The positive control was the pH 4 test tube, and the negative control was the pH 13 test tube. The independent variables were time and pH levels, and the dependent variable was the amount of eaction that occurred.
Each test tube was left at room temperature for one minute and then tested every five seconds for thirty seconds. The technique used was also the same as the first experiment.
This supports my hypothesis that higher temperatures amplify catalyzation, although too high of a temperature denatures enzymes. The lowest reaction was the 4 to 1 ratio, this is because the 3 to 2 and 4 to 1 ratios, too much catalase was added.
The catalase broke down all the hydrogen peroxide and then had a surplus after the reaction occurred. This supports my hypothesis that smaller concentrations of enzyme in the enzyme to substrate concentration ratios produce higher reactions.Enzyme lab report.
Introduction. An enzyme is a protein molecule that speeds up the rates of chemical reactions by many folds. They recognize, bind, and change specific reactants. In enzyme-catalyzed reactions, a substrate, the substance to be acted upon, binds to the active site on an enzyme to form the desired product.
Each active site on the enzyme is unique to the substrate it will bind with causing each to have an individual three-dimensional structure.
The major objective of this experiment was to observe the effects of catalase under varying controlled conditions. The scope of this experiment includes Metabolic processes, such as cellular respiration, and it poisonous byproduct hydrogen peroxide. Enzymes Lab Report Essay example; Enzymes Lab Report Essay example.
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Show More. Enzymes Lab Report Inroduction In this lab we explore an enzymes activity and how it can be affected by changes to its environment.
An enzyme is a protein and is a catalyst to chemical reactions. Essay on Enzyme Lab Report. Enzyme Lab Report Essay. The oxygen can be observed as bubbles comic Eng from the reaction site - Enzyme Lab Report Essay introduction.
Catalane is found in . More Essay Examples on Enzyme Rubric. In agarose gel electrophoresis, a molecular sieve is created such that the distance traveled in the gel toward the anode by any DNA fragment (all of which carry negatively charged phosphate groups in the presence of a basic buffer) is inversely proportional to its molecular weight.